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Plasmablasts |  Early plasma cells |  Bone marrow plasma cells | 

(Please click on each figure of interest to zoom)

Figure 1. Heatmap of the top10 genes overexpressed in each of the 8 B cell to plasma cell subpopulations. The names of top10 genes overexpressed in each cell population are indicated on the right. Each population is highlighted by a color assigned by default by GenomicScape. The red and blue colors on the heatmap indicate over-expressed and under-expressed genes respectively and the color intensity the expression level.

Figure 2. Molecular pathways enriched in the 8 B cell to plasma cell subpopulations. The molecular pathways encoded by the 9036 genes differentially expressed between the 8 cell populations were analyzed with Reactome software and the 2-3 most significant pathways for each population shown.

Figure 3. Principal component analysis of gene expression profiles of the 8 B cell to plasma cell subpopulations. Using SAM multiclass analysis, 9036 unique genes were significantly differentially expressed between the 8 cell populations (fold change ≥ 2 and FDR = 0%). These genes classified the 8 populations into 8 distinct groups using principal component analysis and these 8 populations could be grouped into 5 major clusters: a B-cell cluster including NBCs and MBCs, a GC cluster CBs and CCs, a PB cluster PBs, EPCs, a PrePB cluster including PrePBs only being located between GC and PB clusters, and a mature PC cluster comprising BMPCs. The figure is the 3D plotting of the samples along the first 3 principal components accounting for 36.1%, 25.7% and 14% of the variance respectively. The 5 clusters comprising the populations are shown by colored clouds.

Figure 4. Visualization of genes coding for B cell or plasma cell surface markers. Gene expression was assayed using Affymetrix U133 plus 2.0 microarrays and data are the unlogged GCRMA-normalized expression signal of each gene in the 8 B cell to plasma cell populations. Data are the expression profiles of genes coding for B cell or plasma cell surface markers obtained with GenomicScape.

Figure 5. Visualization of the genes coding for B cell or plasma cell transcription factors. See legend of figure 4. Data are the expression profiles of genes coding for transcription factors controlling B cell or plasma cell identities obtained with GenomicScape.

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